Identification of the glycosylation site of a major soybean allergen, Gly m Bd 30K.
نویسندگان
چکیده
A major soybean allergen, Gly m Bd 30K, is a glycoprotein. A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrolyzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn170 residue. Furthermore, the sugar moiety of the allergen and peptide was shown to consist of mannose, N-acetylglucosamine, fucose, and xylose at a molar ratio of 3 : 2 : 1 : 1. These results indicate that Gly m Bd 30 K is a N-linked glycoprotein.
منابع مشابه
NoteMeasurement of Gly m Bd 30 K , a Major Sandwich Enzyme - linked ImmunosorbentSoybeanAssayAllergen , in Soybean Products
derrnatitis, we found 16 allergenic proteins in the soybean. i) About two-thirds of soybean-sensitive patients examined was shown to have IgE antibodies binding to the 30-kDa protein narned Gly m Bd 30K. This finding suggests that Gly m Bd 30K is a major allergen in the soybean and that it may be a target allergen for hypoallergenization of soybean products. Gly m Bd 30K has been purified to ho...
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عنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 60 2 شماره
صفحات -
تاریخ انتشار 1996